Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: DLX3-Dependent Regulation of Ion Transporters and Carbonic Anhydrases Is Crucial for Enamel Mineralization

doi: 10.1002/jbmr.3022

Figure Lengend Snippet: Altered expression of carbonic anhydrases in the enamel organ from Dlx3K14–cKO mice. (A) Heat map showing log(RPKM) levels of carbonic anhydrases in enamel organs from Dlx3WT and Dlx3K14–cKO mice. Fold-change and significance are indicated. (B) Western blot analysis of DLX3, CA2, CA6, and actin protein levels in the enamel organ (E.O.) and salivary gland (S.G.) of Dlx3WT and Dlx3K14–cKO mice at P10. (C) Immunohistochemical detection of CA2 and CA6 (brown precipitate) in mandibular incisor longitudinal sections from Dlx3WT and Dlx3K14–cKO mice at P15. Secretory (s), transition (t), and maturation (m) stages are indicated. Scale bar = 100 μm. (D) Measurement of carbonic anhydrase activity in extracts from E.O. and S.G. from Dlx3WT and Dlx3K14–cKO mice at P10. (E) ChIP-seq analysis of DLX3 and H3K4me3 DNA binding domains in enamel organ dissected from rat mandibular incisors (continuously growing), showing DLX3 binding near the proximal promoter (arrowhead) of Car2, Car3, Car6, Car12, and Car13, but not Car1. E.O. = enamel organ; S.G. = salivary gland.

Article Snippet: Primary antibodies used: rabbit anti-DLX3 (Abcam, Cambridge, MA, USA), rabbit anti-AMELX (Abcam), rabbit anti-CA2 (GeneTex Inc., Irvine, CA, USA), rabbit anti-CA6 (Abcam).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Activity Assay, ChIP-sequencing, Binding Assay

Journal: JCI insight

Article Title: Improved mitochondrial function in the heart of sarcolipin-deficient dystrophin and utrophin double knockout mice.

doi: 10.1172/jci.insight.170185

Figure Lengend Snippet: Figure 1: SR Ca2+ handling is improved in cardiac myocytes from mdx:utrn-/-:sln+/- mice.

Article Snippet: The membrane strips were then blocked with 3% milk in phosphate-buffered saline (PBS) and probed overnight at 4o C using antibodies specific for SLN (anti-rabbit, 1:3000, custom made) (57), PLN (anti-rabbit, 1:5000, custom made) (57), RyR2 (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-77717), SERCA2a (anti-rabbit, 1:5000, custom made) (57), mitochondrial Ca2+ uniporter (MCU; antirabbit, 1:1000, CST, #149973), mitochondrial Ca2+ uptake 1 (MICU1; anti-rabbit, 1:1000, CST, #12524), NCXL (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-114330), LETM1 (antirabbit, 1:1000, ABclonal, #A15685), TRPC1 (anti-rabbit, 1:1000, Abcam, #ab192031), total oxidative phosphorylation subunits cocktail (OXPHOS subunits; anti-mouse, 1:10000, Abcam, #ab110413), 4-HNE (anti-rabbit, 1:3000, Abcam, #ab46545), SOD2 (anti-mouse,1:1000, Santacruz, #sc-137254), long-chain fatty-acid-coenzyme A ligase 4 (FACL4/ACSL4, anti-mouse, 1:1000, Santacruz, #sc-365230), glucose-regulated protein 75 (Grp75, anti-rabbit, 1:1000, CST, #3593), -tubulin (anti-mouse, 1:5000, Sigma-Aldrich, #T6199), lamin A/C (4C11, anti-mouse, 1:1000, CST, #4777S) and cytochrome c oxidase IV (COX IV; anti-rabbit, 1:1000, CST, #4850T).

Techniques:

Journal: JCI insight

Article Title: Improved mitochondrial function in the heart of sarcolipin-deficient dystrophin and utrophin double knockout mice.

doi: 10.1172/jci.insight.170185

Figure Lengend Snippet: Figure 2: Mitochondrial Ca2+ handling is improved in the mdx:utrn-/-:sln+/- myocardium. (A)

Article Snippet: The membrane strips were then blocked with 3% milk in phosphate-buffered saline (PBS) and probed overnight at 4o C using antibodies specific for SLN (anti-rabbit, 1:3000, custom made) (57), PLN (anti-rabbit, 1:5000, custom made) (57), RyR2 (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-77717), SERCA2a (anti-rabbit, 1:5000, custom made) (57), mitochondrial Ca2+ uniporter (MCU; antirabbit, 1:1000, CST, #149973), mitochondrial Ca2+ uptake 1 (MICU1; anti-rabbit, 1:1000, CST, #12524), NCXL (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-114330), LETM1 (antirabbit, 1:1000, ABclonal, #A15685), TRPC1 (anti-rabbit, 1:1000, Abcam, #ab192031), total oxidative phosphorylation subunits cocktail (OXPHOS subunits; anti-mouse, 1:10000, Abcam, #ab110413), 4-HNE (anti-rabbit, 1:3000, Abcam, #ab46545), SOD2 (anti-mouse,1:1000, Santacruz, #sc-137254), long-chain fatty-acid-coenzyme A ligase 4 (FACL4/ACSL4, anti-mouse, 1:1000, Santacruz, #sc-365230), glucose-regulated protein 75 (Grp75, anti-rabbit, 1:1000, CST, #3593), -tubulin (anti-mouse, 1:5000, Sigma-Aldrich, #T6199), lamin A/C (4C11, anti-mouse, 1:1000, CST, #4777S) and cytochrome c oxidase IV (COX IV; anti-rabbit, 1:1000, CST, #4850T).

Techniques:

Journal: JCI insight

Article Title: Improved mitochondrial function in the heart of sarcolipin-deficient dystrophin and utrophin double knockout mice.

doi: 10.1172/jci.insight.170185

Figure Lengend Snippet: Figure 1: SR Ca2+ handling is improved in cardiac myocytes from mdx:utrn-/-:sln+/- mice.

Article Snippet: The membrane strips were then blocked with 3% milk in phosphate-buffered saline (PBS) and probed overnight at 4o C using antibodies specific for SLN (anti-rabbit, 1:3000, custom made) (57), PLN (anti-rabbit, 1:5000, custom made) (57), RyR2 (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-77717), SERCA2a (anti-rabbit, 1:5000, custom made) (57), mitochondrial Ca2+ uniporter (MCU; antirabbit, 1:1000, CST, #149973), mitochondrial Ca2+ uptake 1 (MICU1; anti-rabbit, 1:1000, CST, #12524), NCXL (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-114330), LETM1 (antirabbit, 1:1000, ABclonal, #A15685), TRPC1 (anti-rabbit, 1:1000, Abcam, #ab192031), total oxidative phosphorylation subunits cocktail (OXPHOS subunits; anti-mouse, 1:10000, Abcam, #ab110413), 4-HNE (anti-rabbit, 1:3000, Abcam, #ab46545), SOD2 (anti-mouse,1:1000, Santacruz, #sc-137254), long-chain fatty-acid-coenzyme A ligase 4 (FACL4/ACSL4, anti-mouse, 1:1000, Santacruz, #sc-365230), glucose-regulated protein 75 (Grp75, anti-rabbit, 1:1000, CST, #3593), -tubulin (anti-mouse, 1:5000, Sigma-Aldrich, #T6199), lamin A/C (4C11, anti-mouse, 1:1000, CST, #4777S) and cytochrome c oxidase IV (COX IV; anti-rabbit, 1:1000, CST, #4850T).

Techniques:

Journal: JCI insight

Article Title: Improved mitochondrial function in the heart of sarcolipin-deficient dystrophin and utrophin double knockout mice.

doi: 10.1172/jci.insight.170185

Figure Lengend Snippet: Figure 2: Mitochondrial Ca2+ handling is improved in the mdx:utrn-/-:sln+/- myocardium. (A)

Article Snippet: The membrane strips were then blocked with 3% milk in phosphate-buffered saline (PBS) and probed overnight at 4o C using antibodies specific for SLN (anti-rabbit, 1:3000, custom made) (57), PLN (anti-rabbit, 1:5000, custom made) (57), RyR2 (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-77717), SERCA2a (anti-rabbit, 1:5000, custom made) (57), mitochondrial Ca2+ uniporter (MCU; antirabbit, 1:1000, CST, #149973), mitochondrial Ca2+ uptake 1 (MICU1; anti-rabbit, 1:1000, CST, #12524), NCXL (anti-rabbit, 1:1000, ThermoFisher Scientific, PA5-114330), LETM1 (antirabbit, 1:1000, ABclonal, #A15685), TRPC1 (anti-rabbit, 1:1000, Abcam, #ab192031), total oxidative phosphorylation subunits cocktail (OXPHOS subunits; anti-mouse, 1:10000, Abcam, #ab110413), 4-HNE (anti-rabbit, 1:3000, Abcam, #ab46545), SOD2 (anti-mouse,1:1000, Santacruz, #sc-137254), long-chain fatty-acid-coenzyme A ligase 4 (FACL4/ACSL4, anti-mouse, 1:1000, Santacruz, #sc-365230), glucose-regulated protein 75 (Grp75, anti-rabbit, 1:1000, CST, #3593), -tubulin (anti-mouse, 1:5000, Sigma-Aldrich, #T6199), lamin A/C (4C11, anti-mouse, 1:1000, CST, #4777S) and cytochrome c oxidase IV (COX IV; anti-rabbit, 1:1000, CST, #4850T).

Techniques: